Invitrogen™ Caspase 8 Polyclonal Antibody

Catalog No.	Quantity
PIPA5120116	100 μL

Catalog No. PIPA5120116 Supplier Invitrogen™ Supplier No. PA5120116

Description

Rabbit Polyclonal Antibody

Positive test controls include: THP-1, Mouse liver. The target is usually found in the following locations: Cytoplasm. Immunogen sequence: QSCLYAIAEE LGSEDLAALK FLCLDYIPHK KQETIEDAQK LFLRLREKGM LEEGNLSFLK ELLFHISRWD LLVNFLDCNR EEMVRELRDP DNAQISPYRV MLFKLSEEVS ELELRSFKFL LNNEIPKCKL EDDLSLLEIF VEMEKRTMLA ENNLETLKSI CDQVNKSLLG KIEDYERSST ERRMSLEGRE ELPPSVLDEM SLKMAELCDS PREQDSESRT SDKVYQMKNK PRGYCLIINN HDFSKAREDI TQLRKMKDRK GTDCDKEALS KTFKELHFEI VSYDDCTANE IHEILEGYQS ADHKNKDCFI CCILSHGDKG VVYGTDGKEA SIYDLTSYFT GSKCPSLSGK PKIFFIQACQ GSNFQKGVPD EAGFEQQNHT LEVDSSSHKN YIPDEADFLL GMATVKNCVS YRDPVNGTWY IQSLCQSLRE RCPQGDDILS ILTGVNYDVS NKDDRRNKGK QMPQPTFTLR KKLFFPP.

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

Specifications

• Antigen: Caspase 8
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.21 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene: Casp8
• Gene Accession No.: O89110, Q14790
• Gene Alias: ALPS2B; apoptosis-related cysteine peptidase; apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP8; CASP-8; caspase 8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase8; caspase-8; Caspase-8 precursor; Caspase-8 subunit p10; Caspase-8 subunit p18; EC 3.4.22.61; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; FLICE; FLJ17672; ICE8; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581
• Gene Symbols: Casp8
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 4-480 of mouse Caspase 8 (NP_0339421).
• Purification Method: Affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12370, 841
• Target Species: Human, Mouse
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov