MilliporeSigma™ Caspr2, Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABN1380MI	100 μL	Unconjugated

Catalog No. ABN1380MI Supplier MilliporeSigma™ Supplier No. ABN1380

Description

Rabbit Polyclonal Antibody

Contactin-associated protein-like 2 (UniProt Q9UHC6; also known as Cell recognition molecule Caspr2) is encoded by the CNTNAP2 (also known as AUTS15, CASPR2, CDFE, KIAA0868) gene (Gene ID 26047) in human. Caspr2 is a neurexin superfamily member that functions as a neuronal cell adhesion protein. Originally identified in myelinating nerves, Caspr2 serves to cluster voltage-gated potassium (Kv) channels at the juxtaparanode, a membrane domain adjacent to the node of Ranvier, where it forms a barrier holding nodal components in place. Caspr2 is found in axons, dendrites, dendritic spines and the soma of mature pyramidal neurons in the central nervous system (CNS). Caspr2 and its partner Contactin 2 are found in the synaptic plasma membranes fraction of the forebrain. In the absence of Caspr2, new synaptic spines are unable to stabilize, resulting in decreased spine density and altered spine morphology as well as downregulated AMPA receptor subunit on the spines in mice. CNTNAP2 gene mutations are linked to autism spectrum disorder (ASD) in human. Likewise, Cntnap2-knockout mice exhibit core autism-like deficits including abnormal social behavior and communication, and behavior inflexibility. Caspr2 is produced with a signal peptide (a.a. 1-27), the removal of which yields the mature transmembrane proein with a large extracellular region (a.a. 28-1262), followed by a transmembrane domain (a.a. 1263-1283) and a cytoplasmic tail (human a.a. 1284-1331 and mouse a.a. 1284-1332). The extracellular region contains an N-terminal Discoidin domain (a.k.a. F5/8 type C domain or C2-like domain; human a.a. 35-181), four Laminin G-like domains (human a.a. 216-368, 401-552, 799-963, 1055-1214), two EGF-like domains (human a.a. 554-591 and 963-1002), and a Fibrinogen C-terminal domain (human a.a. 592-798).

Specifications

• Antigen: Caspr2
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene: CNTNAP2, AUTS15, CASPR2, CDFE, KIAA0868
• Gene Accession No.: Q9UHC6
• Host Species: Rabbit
• Immunogen: Human IgG1 Fc-tagged recombinant human Caspr2 extracellular domain fragment.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_054860
• Test Specificity: Specificity of this polyclonal antiserum was confirmed by the lack of target detection when using tissue samples from Capr2 knock-out mice. This polyclonal antiserum targets extracellular region absent from human Caspr2 spliced isoform 2 (Uniprot Q9UHC6-2).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.