MilliporeSigma™ CASZ1, Guinea Pig, Unlabeled, Polyclonal,

Guinea Pig Polyclonal Antibody

Supplier:  MilliporeSigma™ ABN1727

Catalog No. ABN1727

Description

Integrin alpha-V (UniProt P06756; CD51, Integrin alpha V, Vitronectin receptor subunit alpha) and Integrin beta-5 (UniProt P18084) are encoded by the ITGAV (MSK8, VNRA, VTNR; Gene ID 3685) and the ITGB5 (Gene ID 3693) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit associated in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence present in the extracellular matrix components, such as fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. Integrin alphaVbeta5 is the primary mechanotransducer that mediates lung myofibroblast contraction-induced latent TGF-beta1 activation. Increased alphaVbeta5 expression is reported to be associated with myofibroblast differentiation. Integrin alphaVbeta5 is shown to interact with Thy-1 in an RLD-dependent manner. Thy-1 expression prevents fibroblast contraction-induced latent TGF-beta1 activation and lung myofibroblast differentiation.

Specifications

• Antigen: CASZ1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Casz1, Cst, D4Ertd432e, Kiaa3026, Srg, Znf693
• Host Species: Guinea Pig
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This guinea pig polyclonal antiserum stained only CASZ1-expressing, but not Casz1 gene-knockout cells in retina tissue sections from E17.5 mouse embryons (Courtesy of Pierre Mattar, Christine Jolicoeur, and Michel Cayouette, Institut de recherches cliniques de Montreal (IRCM), Canada). Epitope regions are present in both CASZ1 spliced isoforms/variants (CASZ1V1 and CASZ1V2) of human and mouse protein reported by UniProt (Q86V15 and Q9CWL2). Human spliced isoforms/variants 1 and 2 are also known as hCASZ11 and hCASZ5, respectively.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Guinea Pig polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: Q9CWL2
• Immunogen: A mixture of three peptides representing two internal sequences N-terminal to the first zinc finger and a third sequence within the region between the third and fourth zinc fingers.
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_001152816
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.