Invitrogen™ Catenin alpha-1 Monoclonal Antibody (10I2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549204

Catalog No. PIMA549204

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1. Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human U20S whole cell, human U-87MG whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell. IHC: human mammary cancer tissue. Flow: Jurkat cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Specifications

• Antigen: Catenin alpha-1
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: P26231, P35221
• Gene Symbols: CTNNA1
• Immunogen: E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 10I2
• Conjugate: Unconjugated
• Gene: CTNNA1
• Gene Alias: [a]E-catenin; 102 kDa cadherin-associated protein; 2010010M04Rik; AA517462; AI988031; alpha E catenin; alpha E-catenin; alpha(E)-catenin; alpha-E-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha; catenin (cadherin-associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; catenin alpha 1 S homeolog; catenin alpha-1; catenin, alpha 1; Catna1; cell-adhesion protein alphaE catenin; ctnna; ctnna1; ctnna1.S; MDPT2; Renal carcinoma antigen NY-REN-13; XELAEV_18020018mg
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12385, 1495, 307505
• Content And Storage: -20°C
• Form: Lyophilized