Catenin alpha-1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA125081

Catalog No. PIPA125081

Description

Recommended positive controls: A431 and cytosolic fraction of rat embryonic brain, MDCK, MCF-7. PA1-25081 is expected to cross react with mouse (100% conserved) and Xenopus laevis (100% conserved) due to sequence homology. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

Specifications

• Antigen: Catenin alpha-1
• Classification: Polyclonal
• Formulation: 0.01M PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: P26231, P35221
• Gene Symbols: CTNNA1, ctnna1.S
• Immunogen: Synthetic peptide, conjugated to KLH, corresponding to residues E(873) K Q D E T Q T K I K R A S Q(887) of human alpha 1 Catenin.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Rat, X. laevis
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Western Blot
• Conjugate: Unconjugated
• Gene: CTNNA1
• Gene Alias: [a]E-catenin; 102 kDa cadherin-associated protein; 2010010M04Rik; AA517462; AI988031; alpha E catenin; alpha E-catenin; alpha(E)-catenin; alpha-E-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha; catenin (cadherin-associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; catenin alpha 1 S homeolog; catenin alpha-1; catenin, alpha 1; Catna1; cell-adhesion protein alphaE catenin; ctnna; ctnna1; ctnna1.S; MDPT2; Renal carcinoma antigen NY-REN-13; XELAEV_18020018mg
• Host Species: Rabbit
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 12385, 1495, 307505, 399303, 474698
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov