MilliporeSigma™ Cav1.1 alpha1s, Mouse, Unlabeled, Clone: 1A,

Catalog No.	Quantity	Conjugate
MAB427CMI	100 μg	Unconjugated

Catalog No. MAB427CMI Supplier MilliporeSigma™ Supplier No. MAB427C

Description

Mouse Monoclonal Antibody

Insulin receptor substrate 1 (UniProt P35568; also known as IRS-1) is encoded by the IRS1 gene (Gene ID 3667) in human. The insulin receptor substrates (IRS-1, IRS-2, IRS-3, and IRS-4) are adaptor proteins involved in modulating cell growth, metabolism, survival, and differentiation by transducing receptor signaling to multiple downstream effectors. IRS family proteins contain a conserved N-terminal pleckstrin homology (PH) domain (a.a. 12-115 of human IRS-1), a phosphotyrosine-binding (PTB) domain (a.a. 160-264 of human IRS-1), and multiple tyrosine phosphorylation sites in the C-terminal region, including Tyr612, Tyr896, Tyr941, Tyr1158, and Tyr1220. The PTB domain meidates the interaction of IRS-1, -2 and -3 with IR juxtamembrane NPXYp motif, facilitating subsequent receptor-mediated IRS tyrosine phosphorylation. Upon phosphorylation, IRS proteins serve as binding sites for Src homology 2 (SH2) domain-containing proteins, such as SHP-2 and PI 3-kinase. Functions of IRS proteins are largely dependent on the phosphorylations of specific tyrosine residues, which in turn are subject to negative regulation by phosphatase activity. In addition, IRS proteins can be phosphorylated by serine/threonine kinases, such as PI-3 kinase, Akt/PKB, GSK-3, mTOR, and PKC, and increased Ser/Thr phosphorylation reduces their binding to IR and tyrosine phosphorylation. Inhibition of PI 3-kinase enhances insulin-stimulated IRS-1 Tyr612/Tyr941 phosphorylation (p85-binding sites) and increases IRS-1 association with PI 3-kinase p85 subunit.

Specifications

• Antigen: Cav1.1 alpha1s
• Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 1A
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
• Gene: CACNA1S, CACH1, CACN1, CACNL1A3
• Gene Accession No.: P07293
• Host Species: Mouse
• Immunogen: Dihydropyridine-binding complex isolated from rabbit muscle t-tubules.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001095190
• Test Specificity: Clone 1A targets the extracellular region of voltage-dependent L-type calcium channel subunit alpha-1S or Cav1.1 encoded by the CACNA1S gene.
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.