Invitrogen™ CaV3.2 Monoclonal Antibody (N55/10), PE

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545398

Catalog No. PIMA545398

Description

1 μg/mL of MA5-45398 was sufficient for detection of Cav3.2 in 10 μg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 260kDa. No cross-reactivity against Cav1.3.

This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants, encoding different isoforms, have been characterized for the gene described here. Studies suggest certain mutations in this gene lead to childhood absence epilepsy (CAE).

Specifications

• Antigen: CaV3.2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: O88427, O95180, Q9EQ60
• Gene Symbols: CACNA1H
• Immunogen: Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Immunocytochemistry, Microarray
• Clone: N55/10
• Conjugate: PE
• Gene: CACNA1H
• Gene Alias: A1H-b; alpha13.2; CACNA1H; CACNA1HB; calcium channel alpha13.2 subunit; calcium channel, voltage-dependent, T type, alpha 1H subunit; calcium channel, voltage-dependent, T type, alpha 1Hb subunit; calcium voltage-gated channel subunit alpha1 H; Cav3.2; ECA6; EIG6; FLJ90484; Low-voltage-activated calcium channel alpha1 3.2 subunit; low-voltage-activated calcium channel alpha13.2 subunit; MNCb-1209; T-type Cav3.2; voltage dependent t-type calcium channel alpha-1H subunit; voltage-dependent T-type calcium channel subunit alpha-1H; voltage-gated calcium channel alpha subunit Cav3.2; voltage-gated calcium channel alpha subunit CavT.2; voltage-gated calcium channel subunit alpha Cav3.2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 114862, 58226, 8912
• Content And Storage: 4°C
• Form: Liquid