Invitrogen™ CBF beta Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528505

Catalog No. PIPA528505

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (85%), Xenopus laevis (92%), Cat (100%), Chicken (96%), Rhesus Monkey (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CBF beta
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CBFB
• Gene Alias: AI893578; CBFB; CBF-beta; core binding factor beta; core-binding factor subunit beta; core-binding factor, beta subunit; PEA2; PEA2-beta; Pebp2; PEBP2B; PEBP2-beta; Pebpb2; polyomavirus enhancer binding protein 2, beta subunit; polyomavirus enhancer-binding protein 2 beta subunit; SL3/AKV core-binding factor beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 865
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q13951
• Gene Symbols: CBFB
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 182 of Human PEBP2beta.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov