CCL2/JE/MCP-1 Rat anti-Mouse, Clone: 123616R, R&D Systems™

Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MAB479R100	R&D Systems MAB479R100	100 ug
MAB479RSP	R&D Systems MAB479RSP	25 ug

Description

Mouse CCL2 is a member of the β (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

Specifications

• Antigen: CCL2/JE/MCP-1
• Format: Purified
• Applications: Flow Cytometry
• Host Species: Rat
• Purification Method: Protein A or G purified from cell culture supernatant
• Regulatory Status: RUO

• Formulation: Supplied as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Clone: 123616R
• Conjugate: Unconjugated
• Isotype: IgG2b
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.