Invitrogen™ CCL5 (RANTES) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ P230E

Catalog No. ENP230E

Description

P230E targets RANTES in ELISA, and WB applications and shows reactivity with Human samples. The P230E immunogen is recombinant human RANTES. P230E detects RANTES which has a predicted molecular weight of approximately 8 kDa. The P230E Rantes polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M230B (biotinylated conjugate of clone VL-1) Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Antibody P230E and biotinylated antibody M230B (clone VL-1) have successfully been used in combination with recombinant Rantes protein SRNTS in ELISA applications.

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

Specifications

• Antigen: CCL5 (RANTES)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CCL5
• Gene Alias: beta-chemokine RANTES; CC chemokine; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES; RANTES protein; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; Scya5; SIS d; SISd; SIS-delta; SISd; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; Unknown (protein for MGC:127014)
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 6352
• Content And Storage: -20°C
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P13501
• Gene Symbols: CCL5
• Immunogen: Recombinant human RANTES.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG