CCR5 (CD195), Rat anti-Human, Clone: HEK/1/85a/7a, Millipore Sigma™

Catalog No.	Quantity
MABF200525	25 μg
MABF2005100	100 μg

Catalog No. MABF200525 Supplier MilliporeSigma Supplier No. MABF200525UG

Description

Rat Monoclonal Antibody

Anti-CCR5 (CD195), clone HEK/1/85a/7a, Cat. No. MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry.

C-C chemokine receptor type 5 (UniProt: P51681; also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195) is encoded by the CCR5 (also known as CMKBR5) gene (Gene ID: 1234) in human. CCR-5 is a multi-pass membrane protein that is expressed in memory T-cells, macrophages, and immature dendritic cells, and is upregulated by proinflammatory cytokines. It is also shown to be highly expressed in spleen, thymus, and on THP-1 and KG-1a cells. Lower expression is observed in ovary and lung. CCR-5 serves as a receptor for a number of inflammatory CC-chemokines and subsequently transduces a signal by increasing intracellular calcium levels. It also plays a role in the control of granulocytic lineage proliferation or differentiation. CCR-5 is acts as a co-receptor (CD4 being the primary receptor) of HIV-1 and HIV-2. It also couples to Gi class of G-proteins to inhibit cAMP production and stimulate calcium release. CCR-5 undergoes sulfation on two tyrosine residues in the N-terminal region, which increases efficiency of HIV-1 entry into cells. It also undergoes phosphorylation at C-terminal serine residues following binding to CC chemokines, especially APO-RANTES. Mutations in CCR5 gene have been linked to insulin-dependent diabetes mellitus. (Ref.: Blanpain, C., et al. (2002). Receptors Channels. 8(1);19-31).

Specifications

• Antigen: CCR5 (CD195)
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Monoclonal
• Clone: HEK/1/85a/7a
• Conjugate: Unconjugated
• Dilution: Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).
• Formulation: Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195
• Gene Symbols: CCR5;CMKBR5
• Host Species: Rat
• Immunogen: CHO cells transfected with full length human CCR-5 (CD195).
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Inflammation & Immunology
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000570.1
• Test Specificity: Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG2b κ