Invitrogen™ CD10 Chimeric Recombinant Rabbit Monoclonal Antibody (NL-1)

Catalog No.	Quantity
PIMA548117	200 μg

Catalog No. PIMA548117 Supplier Invitrogen™ Supplier No. MA548117

Description

Rabbit Recombinant Monoclonal Antibody

Specificity: This antibody reacts with human CD10. CD10 is a widely-expressed cell-surface metalloprotease, responsible for the cleavage of multiple peptide hormones at target tissues. CD10 is also a common marker for acute lymphocytic leukaemia.

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA) and neutral endopeptidase (NEP), is a Zn2+-dependent metallo-peptidase with neutral metalloendopeptidase activity. It is a 100 kDa type II transmembrane glycoprotein encoded by a gene that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts, although the coding region remains unaffected by alternative splicing. CD10 is involved in the regulation of chemotactic and inflammatory processes involving neutrophils and plays a role in stromal cell-dependent B lymphopoiesis. It is expressed on immature B cells in adult bone marrow, mature B cells in germinal centers, and cells from patients with chronic myelocytic leukemia (CML). Additionally, CD10 is present on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas. Beyond the hematopoietic compartment, CD10 is highly expressed on the brush border of enterocytes and renal tubules and glomeruli, as well as on breast myoepithelial cells, bile canaliculi, and fibroblasts. CD10 cleaves peptides at the amino side of hydrophobic residues, inactivating several biologically active peptides, including endothelin, glucagon, gastrin, neurotensin, bradykinin, enkephalins, substance P, oxytocin, and others. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie Tooth Disease, highlighting its significance in both normal physiological processes and disease states.

Specifications

• Antigen: CD10 Chimeric
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: NL-1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% ProClin 300
• Gene: Mme
• Gene Accession No.: P08473
• Gene Alias: 6030454K05Rik; Atriopeptidase; C85356; CALLA; CD10; CMT2T; common acute lymphoblastic leukemia antigen; common acute lymphocytic leukemia antigen; Enkephalinase; EPN; membrane metallo endopeptidase; membrane metalloendopeptidase; membrane metallo-endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; MME; NEP; neprilysin; neprilysin-390; neprilysin-411; neutral endopeptidase 24.11; SFE; Skin fibroblast elastase
• Gene Symbols: Mme
• Host Species: Rabbit
• Immunogen: BALB/c mice were immunised with acute lymphocytic leukemia cells from a human leukemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma.
• Purification Method: Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4311
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG κ