Invitrogen™ CD100 Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA547711

Catalog No. PIPA547711

Description

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 4B, and rhSemaphrin 6C is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Specifications

• Antigen: CD100
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Sema4d
• Gene Alias: A8; BB18; C9orf164; CD100; coll-4; GR3; M-Sema G; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema J; Sema4d; Semacl2; SEMAJ; semaphorin 4D; semaphorin H; semaphorin-4D; Semaphorin-C-like 2; semaphorin-J; Semcl2
• Host Species: Sheep
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20354
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: O09126
• Gene Symbols: Sema4d
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100 Phe24-Thr657.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG