Invitrogen™ CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), Alexa Fluor™ 488, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-167-13	Invitrogen™ 53100942	100 Tests

Description

Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form. Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio133-1C6 (133-1C6) antibody has been pre-titrated and tested by flow cytometric of human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Specifications

• Antigen: CD100 (SEMA4D)
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 488
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q92854
• Gene ID (Entrez): 10507
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: eBio133-1C6 (133-1C6)
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgM
• Target Species: Human
• Gene: Sema4d
• Gene Alias: A8; BB18; C9orf164; CD100; coll-4; GR3; M-Sema G; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema J; Sema4d; Semacl2; SEMAJ; semaphorin 4D; semaphorin H; semaphorin-4D; Semaphorin-C-like 2; semaphorin-J; Semcl2
• Gene Symbols: Sema4d
• Product Type: Antibody