Invitrogen™ CD103 Polyclonal Antibody, Alexa Fluor™ 488

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA546876

Catalog No. PIPA546876

Description

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 3, rmIntegrin alpha 4, recombinant human (rh) Integrin alpha M, and rhIntegrin alpha V is observed.

CD103, also known as ITGAE or Integrin alpha E, is a molecule that non-covalently associates with integrin beta 7 to form a heterodimeric integral membrane protein. This protein is part of the integrin family, characterized by an alpha chain and a beta chain. Specifically, CD103 includes a 150 kDa alpha chain and a 120 kDa beta chain, with the alpha integrin undergoing post-translational cleavage in the extracellular domain to yield disulfide-linked heavy and light chains. CD103 is primarily expressed on mucosa-associated T lymphocytes, intraepithelial lymphocytes (IELs), and activated cells, as well as a subset of TGF beta-1 cells. It is preferentially found in human intestinal IELs, where it plays a role in adhesion and may serve as an accessory molecule for IEL activation. CD103 binds to epithelial cell antigen E-cadherin, mediating the homing of lymphocytes to the intestinal epithelium. In peripheral blood and lymphoid organs, CD103 is expressed by 0.5 to 2% of resting lymphocytes, and its expression can be upregulated in vitro by stimulating human T cells with mitogens. CD103 is also expressed by hairy cell leukemia (HCL) and some chronic B cell lymphocytic leukemias. The CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

Specifications

• Antigen: CD103
• Classification: Polyclonal
• Formulation: Buffered saline solution with BSA and 0.09% sodium azide
• Gene Accession No.: Q60677
• Gene Symbols: Itgae
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E/CD103 Phe20-Arg1113 (Ser453Gly).
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 488
• Gene: Itgae
• Gene Alias: A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16407
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid