CD106 Rat anti-Mouse, BB700, Clone: 429 (MVCAM.A), BD Optibuild™

Catalog No.	Quantity
BDB742108	50 μg

Catalog No. BDB742108 Supplier BD Biosciences Supplier No. 742108

Description

Rat Monoclonal Antibody

The 429 monoclonal antibody specifically binds to both the long (ñ110 kDa) transmembrane-spanning form and the truncated (ñ47 kDa) GPI-linked form of vascular cell adhesion molecule-1 (VCAM-1, CD106). CD106 is constitutively expressed on bone marrow stromal cells, myeloid cells, and splenic dendritic cells. Its expression on endothelial cells is upregulated by inflammatory cytokines and in certain pathologic conditions. CD106 expression has also been detected on apoptotic thymocytes, splenocytes, and lymphoid cell lines. VCAM-1 is a counter-receptor for VLA-4 (α4β1 integrin) and LPAM-1 (α4β7 integrin), and the 429 antibody partially blocks VCAM-1-mediated binding functions. Source of the immunogen was the mouse preadipose cell line PA6.

The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.

Specifications

• Antigen: CD106
• Classification: Monoclonal
• Clone: 429 (MVCAM.A)
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Blue 700 (BB700)
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Vcam-1, Vascular cell adhesion molecule 1, Vascular cell adhesion protein 1
• Host Species: Rat
• Immunogen: Mouse preadipose cell line PA6
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ