Invitrogen™ CD107b (LAMP-2) Monoclonal Antibody (GL2A7), APC

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA545169

Catalog No. PIMA545169

Description

1 μg/mL of MA5-45169 was sufficient for detection of LAMP2 in 20 μg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 100-110kDa.

LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).

Specifications

• Antigen: CD107b (LAMP-2)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM Phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: P13473, P17047
• Gene Symbols: LAMP2
• Immunogen: Purified preparation of mouse liver lysosomal membranes.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rabbit
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: GL2A7
• Conjugate: APC
• Gene: LAMP2
• Gene Alias: CD107 antigen-like family member B; CD107b; EGK_20887; Igp96; Lamp II; LAMP2; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; Lamp-2a; Lamp-2b; Lamp-2c; LAMPB; LGP110; LGP-110; LGP-96; LGP-B; lysosomal associated membrane protein 2; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2; Mac3; RP23-193O17.2
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100350946, 16784, 3920
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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