Invitrogen™ CD114 (G-CSFR) Monoclonal Antibody (LMM741), PerCP-eFluor™ 710, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 46114942

Catalog No. 50-113-0777

Description

Description: This LMM741 monoclonal antibody reacts with human CD114, which is also known as granulocyte colony-stimulating factor receptor (G-CSFR). A type I hematopoietin cytokine receptor, CD114 is expressed on granulocytes such as neutrophils, myeloid cells, monocytes, and leukemic cells. This receptor is absent from lymphocytes, erythrocytes, and eosinophils. CD114 can also be detected on non-hematopoietic cells, including neurons, endothelial cells, and cardiomyocytes. Studies demonstrate that expression of CD114 can be downregulated by LPS, TNF, phorbol ester, and G-CSF itself. Binding of G-CSF binding induces homodimerization of the receptor that activates signaling transduction (e.g., the JAK/STAT and Erk1/2 pathways) to mediate cell proliferation, survival, and differentiation. Applications Reported: This LMM741 antibody has been reported for use in flow cytometric analysis. Applications Tested: This LMM741 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass ...

CSF3R is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble.

Specifications

• Antigen: CD114 (G-CSFR)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q99062
• Gene Symbols: CSF3R
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1441
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: LMM741
• Conjugate: PerCP-eFluor 710
• Gene: CSF3R
• Gene Alias: CD114; CD114 antigen; colony stimulating factor 3 receptor; colony stimulating factor 3 receptor (granulocyte); Csf3r; Csfgr; G-CSF receptor; GCSFR; G-CSFR; G-CSF-R; Granulocyte colony-stimulating factor receptor; SCN7
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ