CD114 (G-CSFR), PerCP-eFluor 710, clone: LMM741, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 46114942
CSF3R is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble.Description: This LMM741 monoclonal antibody reacts with human CD114, which is also known as granulocyte colony-stimulating factor receptor (G-CSFR). A type I hematopoietin cytokine receptor, CD114 is expressed on granulocytes such as neutrophils, myeloid cells, monocytes, and leukemic cells. This receptor is absent from lymphocytes, erythrocytes, and eosinophils. CD114 can also be detected on non-hematopoietic cells, including neurons, endothelial cells, and cardiomyocytes. Studies demonstrate that expression of CD114 can be downregulated by LPS, TNF, phorbol ester, and G-CSF itself. Binding of G-CSF binding induces homodimerization of the receptor that activates signaling transduction (e.g., the JAK/STAT and Erk1/2 pathways) to mediate cell proliferation, survival, and differentiation.Applications Reported: This LMM741 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LMM741 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5µL (0.25µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100µL cell sample + 100µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD114, GCSFR, CD114 antigen, G-CSF receptor, G-CSF-R, granulocyte colony-stimulating factor receptor|
|IgG1, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
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