CD116 Monoclonal Antibody (4H1), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-116-937	Invitrogen™ 14116937	2 mg
14-116-995	Invitrogen™ 14116995	10 mg

Description

Description: The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor. The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells. Applications Reported: 4H1 has been reported for use in flow cytometric analysis. Applications Tested: The 4H1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.

Specifications

• Antigen: CD116
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CD116; CD116 antigen; CDw116; colony stimulating factor 2 receptor alpha chain; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage); CSF 2R; CSF2R; Csf2ra; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; Csfgmra; GM CSF R alpha; GM-CSF receptor alpha subunit; GMCSFR; GM-CSFR; GM-CSF-Ra; GM-CSFRalpha; GM-CSF-R-alpha; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony stimulating receptor alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838; SMDP4
• Gene Symbols: CSF2RA
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: CSF2RA

• Clone: 4H1
• Applications: Flow Cytometry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P15509
• Gene ID (Entrez): 1438
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody