Novus Biologicals™ CD117/c-kit Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD117/c-kit.

Specifications

• Assay Range: 23.4375 pg/mL
• Assay Sensitivity: 23.44-1500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD117/c-kit.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD117, CD117 antigen, ckit, C-Kit, EC 2.7.10, EC 2.7.10.1, PBT, piebald trait, Proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, SCFRmast/stem cell growth factor receptor, soluble KIT variant 1, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
• Gene ID (Entrez): 3815
• Gene Symbol: KIT
• Host Species: Mouse/Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL mouse anti-CD117/c-kit monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4).
  Capture Antibody: 0.2 mg/mL of rabbit anti-CD117/c-kit monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 90 ng of recombinant CD117/c-kit.
• Quantity: 5 Plates
• Regulatory Status: RUO
• Research Discipline: B Cell Development and Differentiation Markers, Cancer, Cancer Stem Cells, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid derived Suppressor Cell, Phospho Specific, Protein Kinase, Signal Transduction, Stem Cell Markers, Tyrosine Kinases
• Storage Requirements: Storage is content dependent.
• Target: CD117/c-kit
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/mL is recommended.