CD119 Mouse anti-Human, BUV395, Clone: GIR-208, BD Optibuild™

Catalog No.	Quantity
BDB743032	50 μg

Catalog No. BDB743032 Supplier BD Biosciences Supplier No. 743032

Description

Mouse Monoclonal Antibody

The GIR-208 antibody recognizes the extracellular region of CD119 which is also known as the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γreceptor (IFN-γRα). The functionally active-form of the human IFN-γreceptor consists of two (or more) subunits, with IFN-γRαresponsible for IFN-γbinding and both the IFN-γRαandβchains required for the transduction of biologic responses. The IFN-γreceptorαchain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium. Binding of 125I-labeled GIR-208 antibody to IFN-γRα+ cells is reported to be specifically inhibited in the presence of excess IFN-γ. GIR-208 does not cross react with IFN-γas tested by ELISA. The ability of this antibody to bind to IFN-γreceptors of species other than human has not been determined. The immunogen used to generate this hybridoma was human IFN-γ

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: CD119
• Classification: Monoclonal
• Clone: GIR-208
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Ultraviolet 395
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: IFN- gamma receptor alpha chain
• Host Species: Mouse
• Immunogen: Human IFN-γRα
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov