Invitrogen™ CD11a/CD18 Monoclonal Antibody (24)

Catalog No.	Quantity
PIMA533356	100 μg

Catalog No. PIMA533356 Supplier Invitrogen™ Supplier No. MA533356

Description

Mouse Monoclonal Antibody

Product is supplied at 1 mL (100 μL/mL). This antibody inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2-activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore it prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation. Application Notes: Flow cytometry - This antibody stains the extracellular domain of CD11/CD18. Functional Assay -This antibody acts as an inhibitor. The antibody was functionally tested by inhibition of antigen-specific T cell proliferation and LAK cell cytotoxy, which are LEA-1-dependent, and f-Met-Leu-Phe-stimulated chemotaxis, which is a CR3-dependent process. Positive control: PMN, monocytes, lymphocytes.

CD11/CD18 (LFA-1) are beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their functions. Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+. The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding. The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore the monoclonal antibody 24 prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation.

Specifications

• Antigen: CD11a/CD18
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation
• Classification: Monoclonal
• Clone: 24
• Concentration: 100 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% BSA and no preservative
• Gene: ITGAL
• Gene Accession No.: P05107, P20701
• Gene Alias: antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11a; CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin alpha L; integrin alpha-L; integrin beta chain, beta 2; integrin beta-2; integrin gene promoter; integrin subunit alpha L; integrin subunit beta 2; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGAL; ITGB2; LAD; LCAMB; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte cell adhesion molecule CD18; leukocyte function-associated molecule 1 alpha chain; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; MAC-1; MF17; MFI7
• Gene Symbols: ITGAL, ITGB2
• Host Species: Mouse
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3683, 3689
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1