Invitrogen™ CD11a/CD18 Monoclonal Antibody (CVS9), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA528272

Catalog No. PIMA528272

Description

Mouse anti Horse CD11a/CD18 antibody, clone CVS9 recognizes the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin.

CD11/CD18 (LFA-1) are beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their functions. Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+. The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding. The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore the monoclonal antibody 24 prevents deadhesion of receptor/ligand pairs, possible locking leukocyte integrins in an active conformation.

Specifications

• Antigen: CD11a/CD18
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 1% BSA and 0.09% sodium azide
• Gene Accession No.: 0
• Gene Symbols: ITGAL, ITGB2
• Immunogen: Equine leucocytes.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Horse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: CVS9
• Conjugate: FITC
• Gene: ITGAL
• Gene Alias: antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11a; CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin alpha L; integrin alpha-L; integrin beta chain, beta 2; integrin beta-2; integrin gene promoter; integrin subunit alpha L; integrin subunit beta 2; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGAL; ITGB2; LAD; LCAMB; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte cell adhesion molecule CD18; leukocyte function-associated molecule 1 alpha chain; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; MAC-1; MF17; MFI7
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100056128, 100065508
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
• Form: Liquid