CD11a Mouse anti-Human, Brilliant Violet 510, Clone: G43-25B, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743331

Catalog No. BDB743331

Description

The G43-25B monoclonal antibody specifically binds to CD11a. CD11a is the 180 kD integrin chain which associates with CD18 (integrin 2) to form the heterodimer glycoprotein CD11a/CD18. This is known as the lymphocyte function associated antigen-1 (LFA-1), which is on all leukocytes; and is an adhesion molecule involved in lymphocyte and granulocyte functions. LFA-1 mediates adhesion of lymphoid cells to the vascular endothelium in association with its ligand, the intracellular adhesion molecule-1 (ICAM-1), CD54. Other ligands are ICAM-2 (CD102) and ICAM-3 (CD50).

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: CD11a
• Clone: G43-25B
• Conjugate: Brilliant Violet 510
• Gene Alias: LFA-1α, LFA-1A, LFA1A, Integrin alpha-L, ITGAL
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2a κ

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.