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CD11b Antibody - BSA Free, Novus Biologicals™
SDP

Catalog No. NB11089474T Shop All R&D Systems Products
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Description

Rabbit Polyclonal Antibody has been used in 67 publications

CD11b Polyclonal specifically detects CD11b in Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In-situ Hybridization, Single Cell Western, Dual RNAscope ISH-IHC.

Specifications

Antigen CD11b
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), In Situ Hybridization (ISH), Flow Cytometry, In Situ Hybridization (ISH)
Classification Polyclonal
Concentration 1.00 mg/mL
Conjugate Unconjugated
Dilution Western Blot 2 ug/mL. Use reported in scientific literature (PMID 31082627), Simple Western 1:50, Flow Cytometry reported in scientific literature (PMID 21422470), Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200. Use reported in multiple pieces of scientific literature (PMID 23980916), Immunohistochemistry-Paraffin 1:400. Use reported in scientific literature (PMID 31022918), Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916), In-situ Hybridization reported in scientific literature (PMID 27133471), Flow (Cell Surface) 1:10 - 1:1000, Single Cell Western 1:10, Dual RNAscope ISH-IHC
Formulation PBS with 0.02% Sodium Azide
Gene Accession No. P11215, P05555
Gene Alias antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, Cell surface glycoprotein MAC-1 subunit alpha, Complement component 3 receptor 3 subunit, CR-3 alpha chain, CR3A, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement
Gene Symbols ITGAM
Host Species Rabbit
Immunogen Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]
Molecular Weight of Antigen 127.2 kDa
Purification Method Affinity Purified
Quantity 0.025 mL
Regulatory Status RUO
Research Discipline Breast Cancer, Cancer, Cellular Markers, Cytoskeleton Markers, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Microglia Markers, Myeloid Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 3684
Test Specificity Mouse, rat and human. We have feedback that this antibody does not work in human samples with Western blot. Immunogen has 100% homology to bovine and 88% homology with sheep.
Target Species Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque
Content And Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype IgG
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For Research Use Only

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