CD11c Mouse anti-Human, Brilliant Violet 510, Clone: S-HCL-3, BD Optibuild™

Catalog No.	Quantity
BDB744435	50 μg

Catalog No. BDB744435 Supplier BD Biosciences Supplier No. 744435

Description

Mouse Monoclonal Antibody

The S-HCL-3 monoclonal antibody specifically binds to CD11c which is encoded by ITGAX (Integrin alpha X, complement component 3 receptor 4 subunit). CD11c is a ñ150 kDa type I transmembrane glycoprotein that is expressed on dendritic cells, monocytes, macrophages, granulocytes, NK cells and subsets of B and T cells. CD11c is also present on hairy-cell leukemias and on some acute myeloid leukemias (AML). The S-HCL-3 antibody stains sinus histiocytes of lymph node, tonsil, and spleen; macrophages in the cortex and medulla of thymus; Kupffer cells in liver; elongated intertubular cells in kidney; and alveolar macrophages in lung tissue. In malignant tissue, the antibody stains true histiocytic lymphomas; it does not stain carcinomas or T or B lymphomas. CD11c associates with CD18 (ITGB2/Integrin beta-2) to form the CD11c/CD18 heterodimer which is likewise known as Leukocyte adhesion receptor p150,95 or Complement receptor type 4 (CR4). Ligands for this heterodimeric adhesion receptor include fibrinogen, iC3b, CD23, and CD54 (ICAM-1).

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: CD11c
• Classification: Monoclonal
• Clone: S-HCL-3
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: ITGAX, Integrin alpha-X, AlphaX integrin chain, CR4, Leu M5, p150/95 alpha
• Host Species: Mouse
• Immunogen: Human Hairy-Cell Leukemia Cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ