CD123 Mouse anti-Human, Non-human primate, eFluor® 450, Clone: 7G3, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 48123842
Description: This 7G3 monoclonal antibody reacts with human and non-human primate CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (the common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes. In addition, this molecule can be detected on CD34+CD38- hematopoietic stem cells and acute myeloid leukemic (AML) cells. The 7G3 antibody has been demonstrated to inhibit IL-3-mediated cell proliferation and homing of AML leukemic stem cells. Crossblocking studies suggest that 7G3 recognizes a different epitope from 6H6. The 7G3 antibody has been reported to crossreact to chimpanzee, cynomologous monkey, olive baboon, pigtailed macaque, rhesus macaque, and sooty mangabey. Applications Reported: This 7G3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 7G3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®.eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered. IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD123, CDw123, SUT-1|
|Human, Non-human Primate|
|4° C, store in dark, DO NOT FREEZE!|
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