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CD125 Mouse anti-Human, Brilliant Violet 421, Clone: A14, BD Optibuild™

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BDB743927 50 μg
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Catalog No. BDB743927 Supplier BD Biosciences Supplier No. 743927
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Description

Mouse Monoclonal Antibody

The A14 monoclonal antibody specifically recognizes theαchain of the Interleukin-5 Receptor (IL-5Rα), which is also known as CD125. IL-5Rα is a 55-60 kDatype I transmembrane glycoprotein that is expressed on B1 B cells, eosinophils, basophils and mast cells. TheIL-5Rα chainassociates withCD131 (Common β-chain) whichserves as the signaling common β-chain subunitof the IL-5R complex as well as for the Interleukin-3 (IL-3R) and GM-CSF receptor (GM-CSFR) complexes. IL-5 promotes the growth and differentiation of eosinophil precursors and activates mature eosinophils. Clone A14 was developed using IL-5Rα-transfected cells as immunogen. This antibody binding can be blocked by IL-5.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD125
Classification Monoclonal
Clone A14
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias CD125, IL-5Rα, IL5RA, IL5R
Host Species Mouse
Immunogen IL-5Rα-transfected cells
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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