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CD125 Rat anti-Mouse, BB700, Clone: T21, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 742267

Catalog No. BDB742267


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Description

Description

The T21 monoclonal antibody specifically binds to CD125 which is also known as the alpha subunit of the IL-5 Receptor (IL5RA/IL-5Rα). CD125 is a type I transmembrane protein that belongs to the hemopoietin receptor and Ig gene superfamilies. It is expressed on B cells and eosinophils. CD125 associates with the Common β chain (βc/Bc) subunit, also known as CD131, to form the functional IL-5 Receptor complex. Mouse IL-5 belongs to the hematopoietic growth factor family of cytokines and promotes the growth and differentiation of B cells and eosinophils. Upon binding, the T21 antibody blocks IL-5 binding to its receptor and can inhibit IL-5 induced B-cell proliferation and antibody production.

The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes. It is a polymer-based tandem dye developed exclusively by BD Biosciences. With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter). This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.
Specifications

Specifications

CD125
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Il5r, Il5ra, IL-5RA, IL-5Rα, IL-5R-alpha, IL-5R subunit alpha
Mouse T88-M Cell Line
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BB700 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
T21
BB700
Affinity Purified
Rat
Rat WI, also known as Wistar (outbred) IgG1, λ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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