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CD127 Rat anti-Mouse, Brilliant Violet 421, Clone: A7R34, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 566377

Catalog No. BDB566377


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Description

Description

The A7R34 monoclonal antibody specifically binds to mouse CD127 which is also known as the Inter ). CD127 associates with CD132 ( ) to form a high-affinity signaling IL-7R complex that mediates the biological functions of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. CD127 is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, the earliest thymocyte progenitors, CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, naοve and memory T lymphocytes, + T cells, thymic NK cells, and bone marrow stromal cells. CD127 is downregulated upon activation of naοve T cells and CD127 re-expression identifies effector cells that subsequently differentiate into memory T cells. The A7R34 antibody blocks IL-7 binding to CD127. This antibody has been used in both in vitro and in vivo studies to define the biological roles played by CD127.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
Specifications

Specifications

CD127
0.2mg/mL
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Interleukin-7 receptor alpha chain, IL-7R alpha, IL7RA, IL-7RA, IL-7Rα, Il7
Mouse
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV421 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV421 were
RUO
Primary
Murine
A7R34
Brilliant Violet 421
Affinity Purified
Rat
Rat WI, also known as Wistar (outbred) IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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