Invitrogen™ CD13 Monoclonal Antibody (R3-63)

Catalog No.	Quantity
PIMA516930	250 μg

Catalog No. PIMA516930 Supplier Invitrogen™ Supplier No. MA516930

Description

Rat Monoclonal Antibody

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. This clone has been reported to block mouse APN enzyme activity.

CD13, also known as ANPEP, is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on a wide variety of cell types and tissues. It is prominently found on monocytes and granulocytes in the blood, but not on lymphocytes. Additionally, CD13 is expressed on endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, placenta, and myeloid leukemia cells. As a metalloprotease, CD13 preferentially catalyzes the removal of neutral amino acids from small peptides, thereby activating or inactivating bioactive peptides. It plays a crucial role in various biological processes, including the degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and the adhesion and migration of cells. CD13 is also involved in extracellular matrix degradation, signal transduction, inflammatory responses, regulation of intercellular contact, cell motility, and vascularization. In the context of disease, CD13 is associated with the protection of leukemic cells against apoptosis and is linked to a poor prognosis in carcinomas. Furthermore, CD13 functions as an aminopeptidase enzyme and serves as an alpha receptor for coronavirus.

Specifications

• Antigen: CD13
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: R3-63
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene: ANPEP
• Gene Accession No.: P97449
• Gene Alias: alanyl (membrane) aminopeptidase; alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150); alanyl aminopeptidase; alanyl aminopeptidase, membrane; aminopeptidase; aminopeptidase Ey; Aminopeptidase M; aminopeptidase N; aminopeptidase N.; aminopeptidase N/CD13; Anpep; APDE; Apm; AP-M; APN; AP-N; bAPN; cAPN; Cd13; cluster of differentiation antigen 13 (CD13); fAPN; gp130; gp150; h AP-N; hAPN; kidney aminopeptidase M; kidney Zn peptidase; KZP; Lap1; Lap-1; leucine arylaminopeptidase 1; LOW QUALITY PROTEIN: aminopeptidase N; membrane alanine aminiopeptidase; membrane alanine aminopeptidase; membrane alanyl aminopeptidase; membrane protein p161; Microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; p150; pAPN; PEPN; porcine aminopeptidase N; rAPN; rbAPN; Unknown (protein for MGC:126949)
• Gene Symbols: ANPEP
• Host Species: Rat
• Immunogen: Mouse intestinal APN.
• Purification Method: Protein G
• Quantity: 250 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16790
• Target Species: Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a