Invitrogen™ CD13 Monoclonal Antibody (WM-15 (WM15)), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 47013842

Catalog No. 47-013-842

Description

Description: The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. Applications Reported: This WM-15 antibody has been reported for use in flow cytometric analysis. Applications Tested: This WM-15 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No.

CD13, also known as ANPEP, is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on a wide variety of cell types and tissues. It is prominently found on monocytes and granulocytes in the blood, but not on lymphocytes. Additionally, CD13 is expressed on endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, placenta, and myeloid leukemia cells. As a metalloprotease, CD13 preferentially catalyzes the removal of neutral amino acids from small peptides, thereby activating or inactivating bioactive peptides. It plays a crucial role in various biological processes, including the degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and the adhesion and migration of cells. CD13 is also involved in extracellular matrix degradation, signal transduction, inflammatory responses, regulation of intercellular contact, cell motility, and vascularization. In the context of disease, CD13 is associated with the protection of leukemic cells against apoptosis and is linked to a poor prognosis in carcinomas. Furthermore, CD13 functions as an aminopeptidase enzyme and serves as an alpha receptor for coronavirus.

Specifications

• Antigen: CD13
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15144
• Gene Symbols: ANPEP
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 290
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: WM-15 (WM15)
• Conjugate: APC-eFluor 780
• Gene: ANPEP
• Gene Alias: alanyl (membrane) aminopeptidase; alanyl (membrane) aminopeptidase (aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150); alanyl aminopeptidase; alanyl aminopeptidase, membrane; aminopeptidase; aminopeptidase Ey; Aminopeptidase M; aminopeptidase N; aminopeptidase N.; aminopeptidase N/CD13; Anpep; APDE; Apm; AP-M; APN; AP-N; bAPN; cAPN; Cd13; cluster of differentiation antigen 13 (CD13); fAPN; gp130; gp150; h AP-N; hAPN; kidney aminopeptidase M; kidney Zn peptidase; KZP; Lap1; Lap-1; leucine arylaminopeptidase 1; LOW QUALITY PROTEIN: aminopeptidase N; membrane alanine aminiopeptidase; membrane alanine aminopeptidase; membrane alanyl aminopeptidase; membrane protein p161; Microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; p150; pAPN; PEPN; porcine aminopeptidase N; rAPN; rbAPN; Unknown (protein for MGC:126949)
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ