CD131 Mouse anti-Human, BUV395, Clone: 3D7, BD Optibuild™

Catalog No.	Quantity
BDB743322	50 μg

Catalog No. BDB743322 Supplier BD Biosciences Supplier No. 743322

Description

Mouse Monoclonal Antibody

The 3D7 antibody reacts with CD131, the 120 kD commonβchain (βc) which is shared with the receptor complexes for human granulocyte-macrophage colony stimulating factor (GM-CSFR), interleukin-3 (IL-3R) and interleukin-5 (IL-5R). Together with theαsubunit of either the IL-3R (IL-3Rα), IL-5R (IL-5Rα), or GM- CSFR (GM-CSFRα), the commonβchain forms high-affinity, signaling receptors for human IL-3, IL-5 and GM-CSF, respectively. Cell surfaceβc are expressed by a variety of different cell types including hematopoietic progenitor cells derived from pluripotent stem cells, monocytes, neutrophils, eosinophils, basophils, endothelial cells, fibroblasts, and Langerhans cells. The immunogen used to generate this hybridoma was cells co-transfected with expression vectors which contained cDNA for the human IL-3αandβchains.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: CD131
• Classification: Monoclonal
• Clone: 3D7
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Ultraviolet 395
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: CSF2RB, Cytokine receptor common β, βc, CDw131, IL-3Rβ/IL-5Rβ/GM-CSFRβ
• Host Species: Mouse
• Immunogen: Human CD131 Transfected COS Cells
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ