CD132 Mouse anti-Human, Brilliant Violet 650, Clone: AG184, BD Optibuild™

Catalog No.	Quantity
BDB742573	50 μg

Catalog No. BDB742573 Supplier BD Biosciences Supplier No. 742573

Description

Mouse Monoclonal Antibody

The AG184 monoclonal antibody specifically binds to the 65-70 kDa commonγsubunit (γc) that is shared by the IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21 receptor complexes. Theγc receptor is a type 1 transmembrane glycoprotein that is constitutively expressed by most peripheral T and B lymphocytes, NK cells, monocytes and granulocytes. The cytoplasmic domain of theγc chain plays an important role in cytokine-mediated signal transduction. By immunofluorescent staining and flow cytometric analyses, the AG184 antibody has been shown to specifically recognize humanγc expressed by cell lines including humanγc gene-transfected cell lines which are known to express the humanγc chain. The AG184 antibody can bind theγc chain in the receptors complexed with IL-2, IL-4, or IL-7, indicating that the antibody recognizes an epitope which is distinct from the cytokine binding site of theγc chain.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: CD132
• Classification: Monoclonal
• Clone: AG184
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 650
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: IL2RG, IL-2RG, IL-2Rγ, Common gamma chain, γc, CIDX, SCIDX, SCIDX1, IMD4
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ