Invitrogen™ CD137 (4-1BB) Monoclonal Antibody (17B5), Functional Grade, eBioscience™

Syrian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-144-62	Invitrogen™ 16137185	500 μg

Description

Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays. Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis and functional assays. Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes cultures. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

Specifications

• Antigen: CD137 (4-1BB)
• Concentration: 1 mg/mL
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Syrian Hamster
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P20334
• Gene ID (Entrez): 21942
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 17B5
• Content And Storage: 4°C
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Mouse
• Gene: TNFRSF9
• Gene Alias: 4 1 BB; 41BB; 4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; EGK_00183; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
• Gene Symbols: TNFRSF9
• Product Type: Antibody