CD140b Mouse anti-Human, BUV395, Clone: 28D4, BD Optibuild™

Catalog No.	Quantity
BDB743039	50 μg

Catalog No. BDB743039 Supplier BD Biosciences Supplier No. 743039

Description

Mouse Monoclonal Antibody

Reacts with platelet derived growth factor (PDGF) receptorβ, a 170-190 kDa single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes. PDGF receptors,αandβ, are single glycoproteins with intracellular tyrosine kinase domains. They are structurally similar to M-CSF receptor and CD117 (c-kit). Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils. Functional PDGF is secreted as a disulfide-linked dimer of A and B chains (PDGF-AA, PDGF-BB or PDGF-AB). Binding of divalent PDGF induces receptor dimerization with three possible forms:αα,αβ,ββ. The PDGF receptorα-subunit binds both PDGF A and B chains, whereas the receptorβ-subunit binds only PDGF B chain. Both receptor subunits can stimulate mitogenic responses, only theβsubunit can induce chemotaxis. 28D4 is specific for PDGFRβand does not cross-react with PDGFRα.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: CD140b
• Classification: Monoclonal
• Clone: 28D4
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Ultraviolet 395
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: PDGF Receptor β chain, PDGFRβ, PDGFRB, PDGFR1, PDGFR, PGFRB, JTK12
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ