Invitrogen™ CD146 Monoclonal Antibody (C264), FITC

Catalog No.	Quantity
PIMA528812	1 mL

Catalog No. PIMA528812

Supplier: Invitrogen™ MA528812

Description

Mouse Monoclonal Antibody

CD146 Monoclonal Antibody for Flow

CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is heavily glycosylated, with more than 50% of its mass derived from carbohydrates. CD146 is primarily expressed on endothelial cells at cell-cell junctions, playing a crucial role in cell adhesion and the cohesion of the endothelial monolayer in vascular tissue. In addition to endothelial cells, CD146 is expressed on bone marrow fibroblasts, mesenchymal stromal cells, endometrial stromal cells, and some tumors, particularly melanoma. It has also been observed on a subset of circulating T cells and activated lymphocytes, but not on circulating endothelial cells, as indicated by the absence of other endothelial markers such as CD31 and CD51/61. CD146 functions as a calcium-independent cell adhesion molecule and may also act as a signal transduction molecule. It is involved in the recruitment of Fyn kinase and the subsequent tyrosine phosphorylation of intracellular proteins, which are important for actin cytoskeleton assembly. This signaling role includes triggering tyrosine phosphorylation of FYN and PTK2 and causing a transient increase in intracellular calcium concentration. In the context of cancer, CD146 expression may facilitate the interaction of melanoma cells with the vascular system, enhancing hematogenous tumor spread. Additionally, CD146 could serve as an adhesion molecule active in neural crest cells during embryonic development, highlighting its diverse roles in both normal physiological processes and disease states.

Specifications

• Antigen: CD146
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: C264
• Concentration: 0.5 mg/mL
• Conjugate: FITC
• Formulation: PBS with <0.1% sodium azide; pH 7.4
• Gene: MCAM
• Gene Accession No.: 0
• Gene Alias: 1-gicerin; AV025631; CD146; CD149; Cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; l-gicerin; l-gicerin precursor; l-gicerin protein; LOW QUALITY PROTEIN: cell surface glycoprotein MUC18; MCAM; melanoma adhesion molecule; melanoma cell adhesion molecule; Melanoma-associated antigen A32; melanoma-associated antigen MUC18; Muc18; mucin-18; s-endo; S-endo 1 endothelial-associated antigen; s-gicerin; s-gicerin precursor
• Gene Symbols: MCAM
• Host Species: Mouse
• Immunogen: Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos.
• Purification Method: Purified
• Quantity: 1 mL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 448832
• Target Species: Avian, Chicken
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ