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CD15 Mouse anti-Human, Clone: HI98, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$54.25 - $117.00

Specifications

Antigen CD15
Clone HI98
Target Species Human
Conjugate Unconjugated
Isotype IgM, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-124-36 Non-distribution product as customer accommodation.
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Invitrogen
14015980
25 μg
Each for $54.25
Add to cart
 
50-124-37 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14015982
100 μg
Each for $117.00
Add to cart
 
Description

Description

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative. Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P). Applications Tested: The HI98 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The HI98 antibody has also been tested by immunohistochemistry on FFPE tisue (IHC-P) at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determit (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Specifications

Specifications

CD15
Human
IgM, kappa
Monoclonal
Liquid
Antibody
4° C
HI98
Unconjugated
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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