Invitrogen™ CD151 Monoclonal Antibody (11G5a)

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA180804	Invitrogen™ MA180804	200 μg
PIMA516443	Invitrogen™ MA516443	100 μg

Description

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is tonsil or spleen. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules.

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.

Specifications

• Antigen: CD151
• Concentration: 1 mg/mL
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P48509
• Gene ID (Entrez): 977
• Immunogen: The details of the immunogen for this antibody are not currently available.
• Product Type: Antibody

• Clone: 11G5a
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1
• Target Species: Human
• Gene: CD151
• Gene Alias: CD 151; CD151; CD151 antigen; CD151 antigen (Raph blood group); CD151 molecule; CD151 molecule (Raph blood group); GP27; hemidesmosomal tetraspanin CD151; membrane glycoprotein SFA-1; MER2; Peta3; PETA-3; platelet endothelial tetraspan antigen-3; platelet surface glycoprotein gp27; platelet-endothelial cell tetraspan antigen 3; platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; Tetraspanin-24; TSPAN24; tspan-24
• Gene Symbols: CD151
• Primary or Secondary: Primary
• Purification Method: Protein A