Invitrogen™ CD151 Monoclonal Antibody (50-6), APC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 17151942

Catalog No. 50-150-92

Description

Description: The monoclonal antibody 50-6 recognizes human CD151 also known as PETA-3 (platelet-endothelial cell tetraspan antigen-3). CD151 has a molecular weight of 29 kDa and like other tetraspanin family members, CD151 contains four hydrophobic transmembrane domains, two short cytoplasmic tails, and one small and one large extracellular loop. CD151 is expressed on platelets, megakaryocytes, endothelial cells, at the cell-cell junctions, as well as epithelial cells where it localizes to basolateral surfaces exposed to extracellular matrix. The tetraspanins play a role in cell adhesion and migration; CD151 interacts with integrinalpha3 beta1 and integrinalpha6 beta4. In particular CD151 forms a functional complex with c-Met and integrin alpha3 and alpha6 in human salivary gland cancer cells. Additionally it can interact with CD46. Together these interactions are thought to play a role during early metastasis as a potential positive effector. The 50-6 monoclonal antibody inhibits in vivo metastasis of a human epidermal carcinoma cell line, HEp-3. Applications Reported: This 50-6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 50-6 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but ...

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.

Specifications

• Antigen: CD151
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P48509
• Gene Symbols: CD151
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 977
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 50-6
• Conjugate: APC
• Gene: CD151
• Gene Alias: CD 151; CD151; CD151 antigen; CD151 antigen (Raph blood group); CD151 molecule; CD151 molecule (Raph blood group); GP27; hemidesmosomal tetraspanin CD151; membrane glycoprotein SFA-1; MER2; Peta3; PETA-3; platelet endothelial tetraspan antigen-3; platelet surface glycoprotein gp27; platelet-endothelial cell tetraspan antigen 3; platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; Tetraspanin-24; TSPAN24; tspan-24
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ