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CD151 Mouse anti-Human, Brilliant Violet 510, Clone: 14A2.H1, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742923

Catalog No. BDB742923

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Reacts with platelet-endothelial cell tetraspan antigen-3 (PETA-3), a 27 kD membrane glycoprotein, expressed on platelets, megakaryocytes, lymphocytes (weak), monocytes, endothelial cells and epithelial cells. PETA-3 (CD151) associates withβ1 integrin in certain tissues. This has also been shown with other tetraspan superfamily members, like CD9, CD63 andα5β1. Reports indicate that this association or colocalization of CD151 withβ1 integrin in tissues suggests a functional role of this molecule, however, this role has not been elucidated yet. It has also been reported that antibody 14A2.H1 is capable of platelet activation in vitro.Studies showed that different clones of CD151 monoclonal antibodies display strikingly different patterns of binding to human haemopoietic cells and tissue sections, and that this is due at least in part to the presence of the protein in complexes with different integrins.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.


Aqueous buffered solution containing ≤0.09% sodium azide.
Human M1 Acute Myeloid Leukemia (AML) Cells
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
Brilliant Violet 510
Affinity Purified
Mouse BALB/c IgG1, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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