CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-129-16	Invitrogen™ 14152282	100 μg

Description

Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly. Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4. Applications Reported: The UC10-4B9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported in in vitro functional studies. (Please use Functional Grade purified UC10-4B9, cat. 16-1522, in functional assays.). Applications Tested: The UC10-4B9 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test.

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.

Specifications

• Antigen: CD152 (CTLA-4)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P09793
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: UC10-4B9
• Applications: Flow Cytometry, Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene ID (Entrez): 12477
• Primary or Secondary: Primary
• Content And Storage: 4°C

For Research Use Only.