CD153 Monoclonal Antibody (RM153), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-106-58	Invitrogen™ 12153181	50 μg
50-106-59	Invitrogen™ 12153182	100 μg
50-106-60	Invitrogen™ 12153183	200 μg

Description

Description: The RM153 monoclonal antibody reacts with mouse CD153 (CD30 ligand). This 40 kDa cell surface receptor, a type II transmembrane protein, is a member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation. Expression of CD153 on T cells does not appear to require costimulation through CD28 and is upregulated via TCR engagement and peaks 24-48 hours after activation. Signaling through CD153 is important in costimulation of pre-activated T cells. Applications Reported: The RM153 antibody has been reported for use in flow cytometric analysis. Applications Tested: The RM153 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.

Specifications

• Antigen: CD153
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CD153; CD153 antigen; CD30 antigen ligand; CD30 ligand; Cd30l; CD30-L; Cd30lg; CD30ligand; Tnfsf8; TNLG3A; tumor necrosis factor (ligand) superfamily member 8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand 3A; tumor necrosis factor ligand superfamily member 8; tumor necrosis factor superfamily member 8
• Gene Symbols: Tnfsf8
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Tnfsf8

• Clone: RM153
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P32972
• Gene ID (Entrez): 21949
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.