CD162 Mouse anti-Human, Brilliant Violet 421, Clone: KPL-1, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743478

Catalog No. BDB743478

Description

The KPL-1 monoclonal antibody specifically binds to p-selectin glycoprotein ligand-1 (PSGL-1), a 120-220 kDa, mucin-like type I transmembrane glycoprotein. CD162 is expressed on neutrophils, monocytes, and most lymphocytes. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). This molecule mediates rolling of leukocytes on activated endothelium and the interaction between leukocytes and activated platelets or other leukocytes found at inflammatory sites. It has also been reported that this clone completely inhibits the interaction between P-Selectin and either CD4-T cells or neutrophils.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: CD162
• Clone: KPL-1
• Conjugate: Brilliant Violet 421
• Gene Alias: SELPLG, Selectin P ligand, P-selectin glycoprotein ligand 1, PSGL-1, CLA
• Immunogen: Human PSGL-1 Transfected Cell Line
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov