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  6. Invitrogen™ CD162 (PSGL-1) Monoclonal Antibody (4RA10), Alexa Fluor™ 488, eBioscience™, Invitrogen™ 

Invitrogen™ CD162 (PSGL-1) Monoclonal Antibody (4RA10), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Catalog No. 53162180
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Catalog No. 53-162-180 Supplier Invitrogen™ Supplier No. 53162180
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Description

Rat Monoclonal Antibody

Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L. Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD162 (PSGL-1)
Applications Flow Cytometry
Classification Monoclonal
Clone 4RA10
Concentration 0.5 mg/mL
Conjugate Alexa Fluor 488
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene SELPLG
Gene Accession No. Q62170
Gene Alias CD162; CLA; Cutaneous lymphocyte associated antigen; cutaneous lymphocyte-associated associated antigen; leukocyte cell surface adhesion molecule; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1 propeptide; P-selectin glycoprotein ligand-1; Psgl1; Psgl-1; Selectin P ligand; selectin, platelet (p-selectin) ligand; Selp1; SELPG; Selpl; SELPLG
Gene Symbols SELPLG
Host Species Rat
Immunogen Mouse PSGL-1 human IgG1 fusion protein.
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 20345
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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