CD166 (ALCAM) Monoclonal Antibody (3A6), Super Bright™ 645, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
64-166-841	Invitrogen™ 64166841	25 Tests
64-166-842	Invitrogen™ 64166842	100 Tests

Description

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells. This 1RNM44N antibody will recognize a formaldehyde-fixed epitope. Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Specifications

• Antigen: CD166 (ALCAM)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: activated leukocyte cell adhesion molecule; AI853494; ALCAM; BEN; CD166; CD166 antigen; cell adhesion molecule; DM-GRASP; FLJ38514; HB2; KG-CAM; MEMD; MGC71733; MuSC; Protein DM-GRASP; Protein MEMD; SB-10 antigen; SC1; unnamed protein product
• Gene Symbols: ALCAM
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: ALCAM

• Clone: 3A6
• Applications: Flow Cytometry
• Conjugate: Super Bright 645
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: Q13740
• Gene ID (Entrez): 214
• Isotype: IgG1 κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody