CD169 (Siglec-1) Rat anti-Mouse, APC-eFluor 780, Clone: SER-4, Invitrogen
Rat Monoclonal Antibody
Manufacturer: LIFE TECHNOLOGIES 47575580
The SER-4 monoclonal antibody reacts with mouse CD169, which is also known as Sialoadhesin and Siglec-1. CD169 is a 185-kDa member of the Siglec family of adhesion and endocytic receptors that recognize sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery. This receptor plays a role in cell-cell adhesion, as well as internalization of sialylated virus and bacteria. This endocytic pathway allows CD169+ macrophages to present lipid antigens to iNKT cells, thereby leading to their activation. Studies have also demonstrated direct interaction of CD169 with regulatory T cells inhibits their proliferation. The SER-4 antibody has been reported to have antagonist activity. Applications Reported: This SER-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SER-4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.
|PBS with 0.09% sodium azide; pH 7.2|
|4°C, store in dark, DO NOT FREEZE!|
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