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  6. Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet™ 563, eBioscience™, Invitrogen™ 

Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Ultra Violet™ 563, eBioscience™, Invitrogen™

Catalog No. 365170280
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36-517-0280 25 μg
36-517-0282 100 μg
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Catalog No. 36-517-0280 Supplier Invitrogen™ Supplier No. 365170280
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Description

Rat Monoclonal Antibody

This 1RNM44N mAb recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells. This 1RNM44N antibody will recognize a formaldehyde-fixed epitope. Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. BUV563 is a tandem dye that emits at 564nm and is intended for use on cytometers equipped with an Ultraviolet (355nm) laser. When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions. Excitation: 350nm; Emission: 564nm; Laser: Ultraviolet Laser.

SIGLEC-F is a sialic acid-binding immunoglobulin-like lectin predominantly expressed on eosinophils in mice, where it serves as a functional paralog of human SIGLEC-8. It acts as an inhibitory receptor with ITIM motifs that recognize sialylated glycans and can induce eosinophil apoptosis, thereby helping regulate allergic inflammation and immune homeostasis.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD170 (Siglec F)
Applications Flow Cytometry
Classification Monoclonal
Clone 1RNM44N
Concentration 0.2 mg/mL
Conjugate Brilliant Ultraviolet 563
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Siglecf
Gene Accession No. Q920G3
Gene Alias SIGLEC-8
Gene Symbols Siglecf
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 233186
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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