Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 14170295

Catalog No. 14-170-295

Description

This 1RNM44N MAb recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells. This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

Specifications

• Antigen: CD170 (Siglec F)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q920G3
• Gene Symbols: Siglecf
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 233186
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunocytochemistry
• Clone: 1RNM44N
• Conjugate: Unconjugated
• Gene: Siglecf
• Gene Alias: CD170; CD33 antigen-like 2; CD33L2; mSiglec-F; OB-binding protein 2; OBBP2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig like lectin 5; sialic acid binding Ig-like lectin 5; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding immunoglobulin-like lectin 5; sialic acid-binding lectin Siglec-F; Siglec; SIGLEC5; siglec-5; Siglecf; Siglec-F
• Host Species: Rat
• Quantity: 10 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ